三个阶段牦牛睾丸发育过程中piRNA的鉴定及分析研究

doi:10.11843/j.issn.0366-6964.2019.11.007

Abstract

Abstract: This research was conducted to identify and analyze piRNA in the testicular development of yaks of different ages. A total of 6 healthy male yaks (two in each group) in the fetal (4-5 months old), calves (1 year old) and juvenile (3 years old) stages were selected. Testis were separated for piRNA sequencing and two samples from yak at the same age were considered as biological duplications. The number, base preference, source and function of piRNA, as well as the chromosome distribution and expression levels of piRNA clusters in the samples were analyzed. The expression of PIWI gene family(PIWIL1-PIWIL4) in the testis of yak at 3 stages were detected by fluorescence quantitative PCR. The results showed that the number of piRNAs in testis of yaks in calves and juvenile was significantly more than that in fetal stage (P<0.01). piRNAs from yak testis showed a strong preference for uracil (U) at their 5' ends, and the most of the piRNA came from the other regions than the intergenic and gene regions. More than 60% piRNA clusters in fetal testes were at low abundance, while more than 70% clusters in testes of calves and juvenile yaks were at high abundance. The expressions of PIWIL1 and PIWIL4 in fetal testes were significantly different compared with those in the other two stages (P<0.05). GO function analysis result showed that the number of piRNA source genes ranked first in biological process, cell component and molecular function were metabolic process, cell and binding, respectively. The structure, function and source of piRNA in yak testes were specific. The number and expression abundance of piRNA in the post-embryonic stage (calves and juvenile) was significantly different from that in the fetal stage, which may be related to the expression difference of PIWIL1 and PIWIL4 between two stages.This research provides a theoretical basis for further studying the mechanism of piRNA regulating yak testicular development and improving yak production performance.

CLC Number:

S823.85

YIN Shi, QIN Wenchang, WANG Bin, ZHOU Jingwen, YANG Liuqing, LI Jian. Identification and Analysis of Relevant piRNA during Testicular Development in Yak of Three Stages[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(11): 2235-2243.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2019.11.007

https://www.xmsyxb.com/EN/Y2019/V50/I11/2235

References 30

[1]	韩杰,熊显荣,蔡雯祎,等.牦牛KDM4A基因克隆、组织表达谱及其在卵母细胞和颗粒细胞中的表达[J].畜牧兽医学报, 2018,49(2):291-299.
	HAN J,XIONG X R,CAI W Y,et al.Cloning of KDM4A gene and its expression in different tissues,oocyte and granulosa cell of yak[J].Acta Veterinaria et Zootechnica Sinica,2018, 49(2):291-299.(in Chinese)
[2]	蔡雯祎,熊显荣,陈通,等.KDM2B基因克隆及其在牦牛组织和卵母细胞减数分裂过程中的表达研究[J].畜牧兽医学报, 2018,49(3):534-541.
	CAI W Y,XIONG X R,CHEN T,et al.Molecular cloning of KDM2B gene and its expression pattern in tissues and the process of oocyte meiosis in yak[J].Acta Veterinaria et Zootechnica Sinica,2018,49(3):534-541.(in Chinese)
[3]	LI B,HE X L,ZHAO Y P,et al.Identification of piRNAs and piRNA clusters in the testes of the Mongolian horse[J].Sci Rep,2019, 9:5022.
[4]	CHALBATANI G M,DANA H,MEMARI F,et al.Biological function and molecular mechanism of piRNA in cancer[J].Pract Lab Med,2019,13:e00113.
[5]	STURM Á,PERCZEL A,IVICS Z,et al.The Piwi-piRNA pathway:road to immortality[J].Aging Cell,2017,16(5):906-911.
[6]	HAN B W,ZAMORE P D.piRNAs[J].Curr Biol,2014,24(16):R730-R733.
[7]	ROSENKRANZ D.piRNA cluster database:a web resource for piRNA producing loci[J].Nucleic Acids Res,2016,44(D1):D223-D230.
[8]	HUANG Y,BAI J Y,REN H T.piRNAs biogenesis and its functions[J].Bioorg Khim,2014,40(3):320-326.
[9]	CZECH B,HANNON G J.One loop to rule them all:the ping-pong cycle and piRNA-guided silencing[J].Trends Biochem Sci, 2016,41(4):324-337.
[10]	HAN B W,WANG W,LI C J,et al.piRNA-guided transposon cleavage initiates Zucchini-dependent,phased piRNA production[J]. Science,2015,348(6236):817-821.
[11]	HAYASHI R,SCHNABL J,HANDLER D,et al.Genetic and mechanistic diversity of piRNA 3'-end formation[J].Nature, 2016,539(7630):588-592.
[12]	GRIVNA S T,BEYRET E,WANG Z,et al.A novel class of small RNAs in mouse spermatogenic cells[J].Genes Dev,2006, 20(13):1709-1714.
[13]	WATANABE T,TAKEDA A,TSUKIYAMA T,et al.Identification and characterization of two novel classes of small RNAs in the mouse germline:retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes[J].Genes Dev,2006,20(13):1732-1743.
[14]	MOYANO M,STEFANI G.piRNA involvement in genome stability and human cancer[J].J Hematol Oncol,2015,8:38.
[15]	GIRARDI E,MIESEN P,PENNINGS B,et al.Histone-derived piRNA biogenesis depends on the ping-pong partners Piwi5 and Ago3 in Aedes aegypti[J].Nucleic Acids Res,2017,45(8):4881-4892.
[16]	NEWKIRK S J,LEE S,GRANDI F C,et al.Intact piRNA pathway prevents L1 mobilization in male meiosis[J].Proc Natl Acad Sci U S A,2017,114(28):E5635-E5644.
[17]	WU S R,GUO W,YAN T,et al.Spermatozoal mRNAs expression implicated in embryonic development were influenced by dietary folate supplementation of breeder roosters by altering spermatozoal piRNA expression profiles[J].Theriogenology, 2019,138:102-110.
[18]	DAI X Y,SHU Y Q,LOU Q Y,et al.Tdrd12 is essential for germ cell development and maintenance in Zebrafish[J].Int J Mol Sci,2017,18(6):1127.
[19]	MANAKOV S A,PEZIC D,MARINOV G K,et al.MIWI2 and MILI have differential effects on piRNA biogenesis and DNA methylation[J].Cell Rep,2015,12(8):1234-1243.
[20]	THÉRON E,MAUPETIT-MEHOUAS S,POUCHIN P,et al.The interplay between the Argonaute proteins Piwi and Aub within Drosophila germarium is critical for oogenesis,piRNA biogenesis and TE silencing[J].Nucleic Acids Res,2018,46(19):10052-10065.
[21]	CANNARELLA R,CONDORELLI R A,DUCA Y,et al.New insights into the genetics of spermatogenic failure:a review of the literature[J].Hum Genet,2019,138(2):125-140.
[22]	SUTHERLAND J M,SOBINOFF A P,FRASER B A,et al.RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis[J].J Cell Physiol,2018,233(4):3262-3273.
[23]	LI Y,LI J L,FANG C C,et al.Genome-wide differential expression of genes and small RNAs in testis of two different porcine breeds and at two different ages[J].Sci Rep,2016,6:26852.
[24]	ZHANG Y,WANG X H,KANG L.A k-mer scheme to predict piRNAs and characterize locust piRNAs[J].Bioinformatics, 2011,27(6):771-776.
[25]	徐璐.鸡精子发生过程中关键piRNAs及Piwill基因功能初步研究[D].扬州:扬州大学,2017.
	XU L.Prelimianary study on the biological function of piRNAs and Piwil1gene during spermatogenesis in poultry[D].Yangzhou:Yangzhou University,2017.(in Chinese)
[26]	王标,付绍印,何小龙,等.绵羊睾丸及卵巢组织中piRNA表达情况的测序分析[C]//2017年全国养羊生产与学术研讨会暨养羊学分会第七次全国会员代表大会.石家庄:中国畜牧兽医学会养羊学分会,2017.
	WANG B,FU S Y,HE X L,et al.Sequencing analysis of the expression of piRNAs in the testes and ovaries of sheep[C]//2017 National Seminar on Sheep Production and Academic Research and the Seventh National Congress of the Society of Sheep Science.Shijia-zhuang:Society of Sheep Breeding, Chinese Society of Animal Husbandry and Veterinary Medicine,2017.(in Chinese)
[27]	FUJIHARA Y,OJI A,LARASATI T,et al.Human globozoospermia-related gene Spata16 is required for sperm formation revealed by CRISPR/Cas9-mediated mouse models[J].Int J Mol Sci,2017,18(10):2208.
[28]	SINGH A P,RAJENDER S.CatSper channel,sperm function and male fertility[J].Reprod BioMed Online,2015,30(1):28-38.
[29]	YAMTICH J,HEO S J,DHAHBI J,et al.piRNA-like small RNAs mark extended 3'UTRs present in germ and somatic cells[J].BMC Genomics,2015,16:462.
[30]	STAUB C,JOHNSON L.Review:Spermatogenesis in the bull[J].Animal,2018,12(S1):S27-S35.

Identification and Analysis of Relevant piRNA during Testicular Development in Yak of Three Stages

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 30

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Yili, TANG Jiao, MIN Qi, YANG Lu, WANG Zening, HU Lian, ZHAO Di, JIANG Mingfeng. Mining Key Candidate Genes of Development and Metabolism in Yak Abomasum Based on Transcriptome Data [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 153-168.
[2]	YAO Ying, ZHOU Yingcong, DU Peiyan, LI Yijuan, QIAN Wenjie, LI Liuyang, YU Zhipeng, CUI Yan, YU Sijiu, FAN Jiangfeng. Proteomic Analysis of Yak Serum During Pregnancy Based on TMT Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 192-206.
[3]	ZHANG Ying, YUAN Ligang, CHEN Guojuan, ZHANG Fang, YANG Dapeng. Analysis of Differential Expression of ET-1/eNOS in the Development of Cryptorchidism in Yak [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 207-217.
[4]	ZHANG Qian, CUI Yan, YU Sijiu, HE Junfeng, PAN Yangyang, WANG Meng. Transcriptome Analysis of the Cerebral Cortex in Newborn and Adult Yaks (Bos grunniens) [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4605-4614.
[5]	WANG Jingyu, PAN Yangyang, XU Gengquan, ZHANG Rui, ZHANG Wenlan, WANG Xiaoshan, WU Rentaodi, ZHAO Rigetu, CUI Yan, YU Sijiu. Preparation and Preliminary Application of Yak (Bos Grunniens) Fas-associated Factor 1 Polyclonal Antibody [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3369-3382.
[6]	ZHANG Peng, WANG Mingxiu, JING Kemin, PENG Wei, TIAN Yuan, LI Yuqian, FU Changqi, SHU Shi, ZHONG Jincheng, CAI Xin. Abnormal Expression of FGFs/FGFRs and Their Mediated Signaling Pathway Genes Affect the Proliferative Activity of Undifferentiated Spermatogonia in Cattleyak [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2886-2897.
[7]	JIAO Zhengxing, PAN Yangyang, WANG Meng, WANG Jinglei, MA Wenbin, GAO Xiang, ZHANG Hui, CUI Yan, YU Sijiu, WANG Libin. Preparation of Polyclonal Antibody against Yak LC3B Protein and Its Application in Detection of Expression in Reproductive Organs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2436-2447.
[8]	MENG Zhaoyi, WANG Yunlu, XU Yefen, NIU Jiaqiang, SUOLANG Sizhu, GUO Min, XI Guangyin. Construction of Yak lncRNA ENSBGRT00000000387.1 Lentivirus Vector and Its Effect on Apoptosis of Yak Follicular Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1058-1070.
[9]	GONG Sanni, JIANG Xudong, MA Yao, LU Jianyuan, ZI Xiangdong. Cloning, Tissue Expression and Protein Localization of FHL2 Gene in Yaks [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 146-156.
[10]	WANG Libin, WANG Meng, SUN Ying, CHEN Rui, ZHANG Tiantian, HUANG Zhenhua, ZHANG Qian, YU Sijiu, PAN Yangyang. CYP19A1 Promotes Autophagy and Early Developmental Ability of Yak Oocytes by Regulating the Levels of Endogenous Estradiol [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4283-4295.
[11]	TANG Jiao, XIA Guo, LIU Yili, MIN Qi, RAN Rong, XIE Shuqiong, MA Shilong, JIANG Mingfeng. The Research of Histomorphological and Transcriptomic of Yak Rumen at Different Ages [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3797-3810.
[12]	MA Xiaoling, PENG Wei, SHU Shi. Serum Dynamic Metabonomics Analysis of Yaks in Late Perinatal Period Based on LCMS Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3811-3826.
[13]	RAN Hongbiao, WANG Hui, CHAI Zhixin, WANG Jiabo, ZHANG Ming, CAI Xin, ZHONG Jincheng. miR-138 Regulates Proliferation and Differentiation of Intramuscular Preadipocyte by Targeting PGC-1α in Yak [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3434-3447.
[14]	TANG Ziwen, CHENG Huaqin, LIU Dongju, PHAGMO Droma, YANG Xue, LI Jian, YIN Shi. The Protective Effect of Proanthocyanidins on Oxidative Damage of Yak Granulosa Cells Induced by Zearalenone [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3006-3017.
[15]	ZHANG Lan, YAO Yifan, QIN Anqi, LI Rui, ZHANG Yiyang, CHEN Shuwu, XU Jin, QIAO Zilin, YANG Kun. Proteomic Analysis for Yaks PASMCs under Hypoxia and Normoxia Conditions Based on TMT Technique [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2182-2193.